Optimization of nutrient medium composition to increase biomass of propionic acid bacteria and acidophilic bacteria

New medium for isolating iron-oxidizing and heterotrophic acidophilic bacteria from acid mine drainage.

A new solid medium is described for growing iron and heterotrophic bacteria from acid mine drainage (AMD). Examination of AMD from five states revealed several kinds of colonies of iron-oxidizing bacteria: (i) smooth, (ii) smooth with secondary growth sectors or branching, (iii) star-shaped, (iv) radiating lobe, and (v) flat-rough. All AMD samples yielded whitish colonies that could not use fer...

Antimicrobial Effect of Lactic Acid Bacteria against Common Pathogenic Bacteria

Abstract       Background and Objective: Probiotics are living microorganisms that have beneficial effects on the health of digestive system. The aim of this study was to evaluate the antimicrobial ability of acidic and neutral supernatants (culture supernatant) of lactic acid bacteria against common bacterial pathogens.        Meth...

Bioassay-Guided Isolation and Characterisation of Antifungal Metabolites Studies of Lactic Acid Bacteria and Propionic Acid Bacteria

Acidophilic, heterotrophic bacteria of acidic mine waters.

Obligately acidophilic, heterotrophic bacteria were isolated both from enrichment cultures developed with acidic mine water and from natural mine drainage. The bacteria were grouped by the ability to utilize a number of organic acids as sole carbon sources. None of the strains were capable of chemolithotrophic growth on inorganic reduced iron and sulfur compounds. All bacteria were rod shaped, ...

Cell wall composition of lactic acid bacteria.

After the discovery of methods for the isolation of bacterial cell walls (l), it was established that walls of gram-positive organisms contain relatively few amino acids. The most frequently occurring and abundant of these are alanine, glutamic acid, aspartic acid, lysine, and diaminopimelic acid. Walls of grampositive organisms also contain large amounts of sugars and amino sugars and are rela...